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cdc12(aa20–40)  (GenScript corporation)

 
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    GenScript corporation cdc12(aa20–40)
    Cdc12(Aa20–40), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdc12(aa20–40)/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    cdc12(aa20–40) - by Bioz Stars, 2026-02
    90/100 stars

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    (A) Top: electrostatic surface potential of the Cdc15 F-BAR domain cytosolic face. Red = negative, blue = positive surface charge. Middle: ribbon diagram of the same face. Bottom: close-up of residues mutated in Cdc15-3A. (B) In vitro binding assays with biotin-labeled Cdc12 peptide (aa 20–40) and purified Cdc15 F-BAR domain (left; wild type and 3A) or Imp2 F-BAR domain (right). F-BAR domain associated with streptavidin resin (PD, pull down) was analyzed by SDS-PAGE followed by Coomassie staining. Cdc12(aa 20–40 <t>P31A)</t> was a negative control . (C) Representative ITC experiment with Cdc12 (aa 20–40) peptide and Cdc15 F-BAR domain (E30K E152K). Top panel shows raw data; bottom panel shows normalized integrated data. See also .
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    (A) Top: electrostatic surface potential of the Cdc15 F-BAR domain cytosolic face. Red = negative, blue = positive surface charge. Middle: ribbon diagram of the same face. Bottom: close-up of residues mutated in Cdc15-3A. (B) In vitro binding assays with biotin-labeled Cdc12 peptide (aa 20–40) and purified Cdc15 F-BAR domain (left; wild type and 3A) or Imp2 F-BAR domain (right). F-BAR domain associated with streptavidin resin (PD, pull down) was analyzed by SDS-PAGE followed by Coomassie staining. Cdc12(aa 20–40 <t>P31A)</t> was a negative control . (C) Representative ITC experiment with Cdc12 (aa 20–40) peptide and Cdc15 F-BAR domain (E30K E152K). Top panel shows raw data; bottom panel shows normalized integrated data. See also .
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    Image Search Results


    (A) Top: electrostatic surface potential of the Cdc15 F-BAR domain cytosolic face. Red = negative, blue = positive surface charge. Middle: ribbon diagram of the same face. Bottom: close-up of residues mutated in Cdc15-3A. (B) In vitro binding assays with biotin-labeled Cdc12 peptide (aa 20–40) and purified Cdc15 F-BAR domain (left; wild type and 3A) or Imp2 F-BAR domain (right). F-BAR domain associated with streptavidin resin (PD, pull down) was analyzed by SDS-PAGE followed by Coomassie staining. Cdc12(aa 20–40 P31A) was a negative control . (C) Representative ITC experiment with Cdc12 (aa 20–40) peptide and Cdc15 F-BAR domain (E30K E152K). Top panel shows raw data; bottom panel shows normalized integrated data. See also .

    Journal: Cell reports

    Article Title: Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis

    doi: 10.1016/j.celrep.2020.108526

    Figure Lengend Snippet: (A) Top: electrostatic surface potential of the Cdc15 F-BAR domain cytosolic face. Red = negative, blue = positive surface charge. Middle: ribbon diagram of the same face. Bottom: close-up of residues mutated in Cdc15-3A. (B) In vitro binding assays with biotin-labeled Cdc12 peptide (aa 20–40) and purified Cdc15 F-BAR domain (left; wild type and 3A) or Imp2 F-BAR domain (right). F-BAR domain associated with streptavidin resin (PD, pull down) was analyzed by SDS-PAGE followed by Coomassie staining. Cdc12(aa 20–40 P31A) was a negative control . (C) Representative ITC experiment with Cdc12 (aa 20–40) peptide and Cdc15 F-BAR domain (E30K E152K). Top panel shows raw data; bottom panel shows normalized integrated data. See also .

    Article Snippet: Biotin-Cdc12(aa20–40) P31A , Genscript , N/A.

    Techniques: In Vitro, Binding Assay, Labeling, Purification, SDS Page, Staining, Negative Control

    (A) Left: representative live-cell images; deconvolved max projections are shown. Right: quantification of mean Cdc12-mNG CR intensity. Error bars represent SEM. ****p < 0.0001, ***p < 0.001, NS, not significant; p > 0.99; one-way ANOVA with Tukey’s post hoc test for multiple comparisons. Total number of cells (n) from three experiments is indicated. Scale bar = 2 µm. (B) Left: DIC images of the indicated strains grown at 25°C. Single Z slices are shown, and scale bar = 5 µm. Right: quantification of morphology defects in indicated strains. Wild type, n = 305 cells; cdc12-P31A , n = 341 cells; cdc15-3A , n = 436 cells; three experiments, error bars = SEM. (C) Representative montages from time-lapse imaging. Minutes elapsed since spindle pole body separation are indicated. Max projections are shown. Scale bar = 2 µm. (D) Quantification of the mean duration of CR events, related to (C). ****p < 0.0001, Student’s t test. Error bars represent SEM, and number of cells analyzed (n) is indicated. (E) Example montage of cdc15-3A cell with aberrant CR constriction. Ellipses denote frames removed for space, and max projections are shown. Scale bar = 2 µm.

    Journal: Cell reports

    Article Title: Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis

    doi: 10.1016/j.celrep.2020.108526

    Figure Lengend Snippet: (A) Left: representative live-cell images; deconvolved max projections are shown. Right: quantification of mean Cdc12-mNG CR intensity. Error bars represent SEM. ****p < 0.0001, ***p < 0.001, NS, not significant; p > 0.99; one-way ANOVA with Tukey’s post hoc test for multiple comparisons. Total number of cells (n) from three experiments is indicated. Scale bar = 2 µm. (B) Left: DIC images of the indicated strains grown at 25°C. Single Z slices are shown, and scale bar = 5 µm. Right: quantification of morphology defects in indicated strains. Wild type, n = 305 cells; cdc12-P31A , n = 341 cells; cdc15-3A , n = 436 cells; three experiments, error bars = SEM. (C) Representative montages from time-lapse imaging. Minutes elapsed since spindle pole body separation are indicated. Max projections are shown. Scale bar = 2 µm. (D) Quantification of the mean duration of CR events, related to (C). ****p < 0.0001, Student’s t test. Error bars represent SEM, and number of cells analyzed (n) is indicated. (E) Example montage of cdc15-3A cell with aberrant CR constriction. Ellipses denote frames removed for space, and max projections are shown. Scale bar = 2 µm.

    Article Snippet: Biotin-Cdc12(aa20–40) P31A , Genscript , N/A.

    Techniques: Imaging

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis

    doi: 10.1016/j.celrep.2020.108526

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Biotin-Cdc12(aa20–40) P31A , Genscript , N/A.

    Techniques: Virus, Recombinant, Purification, Magnetic Beads, Protease Inhibitor, Mutagenesis, Software